For Research Use Only. 1994; 264(5157):436-440. Careers. Temperature: 24C, Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980), Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980), Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980). Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. XP is a registered trademark of Cell Signaling Technology, Inc. Comparisons are not made against non-BD technologies, unless otherwise noted. representative of CST, are rejected and are of no force or effect. 1995; 55(3):505-509. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. Visit our. Flow cytometric analysis of cell cycle changes (B) and the apoptosis (C) after transfection of 293T cells with empty control vector (pWPXLD), wild type p16 or mutant p16 expression plasmids. Flow Cytometry: HeLa cells. Mrton M,Tihanyi N,Gyulavri P,Bnhegyi G,Kapuy O. Degradation of Caytaxin Causes Learning and Memory Deficits via Activation of DAPK1 in Aging. As p16 INK4a mRNA is readily detected in murine or human peripheral blood T cells and increases with aging . Yeager et al. compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or An official website of the United States government. 1994; 265(5177):1364-1365. p16 inhibits cdk4 dependent phosphorylation of the tumor suppressor retinoblastoma protein (Rb) and Rb related proteins, p107 and p130. Nomanbhoy TK,Sharma G,Brown H,Wu J,Aban A,Vogeti S,Alemayehu S,Sykes M,Rosenblum JS,Kozarich JW. Functional analyses of coronary artery disease associated variation on chromosome 9p21 in vascular smooth muscle cells. All rights reserved. Explore pathways related to this product. antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results. Bergeron C, Ronco G, Reuschenbach M, Wentzensen N, Arbyn M, Stoler M, von Knebel Doeberitz M. Int J Cancer. Comparisons are not made against non-BD technologies, unless otherwise noted. Huang W,Zhong W,Xu J,Su B,Huang G,Du J,Liu Q. Patient-derived bladder cancer xenografts in the preclinical development of novel targeted therapies. If the cells now have those . It is listed to be a member of the CDKN2 cyclin-dependent kinase inhibitor family. Link to hereditary melanoma brightens mood for p16 gene. P16 is a reported synonym of the human protein 'cyclin dependent kinase inhibitor 2A', encoded by the gene CDKN2A. No. The full protein is reported to be 156 amino acid residues in length. Any use of Product for diagnostic, This gene generates several transcript variants which differ in their first exons. Heart and circulatory physiology, Li Y,Peng Z,Wang C,Li L,Leng Y,Chen R,Yuan H,Zhou S,Zhang Z,Chen AF. Product name Anti-CDKN2A/p16INK4a antibody [2D9A12] See all CDKN2A/p16INK4a primary antibodies Description Mouse monoclonal [2D9A12] to CDKN2A/p16INK4a Host species Mouse Tested applications Suitable for: Flow Cyt, IHC-P more details Unsuitable for: WB Species reactivity Reacts with: Rat, Human Immunogen In cross-sectional studies, age- and viral genotype-dependent expression of p16INK4A was investigated, and optimal diagnostic parameter cut-offs (using colposcopy and biopsy as a gold standard) were obtained. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Journal of assisted reproduction and genetics, Yan P,Xu J,Zeng Y,Dong G,Cao H,Zheng M,Zhu H. Knockout of 4.1B triggers malignant transformation in SV40T-immortalized mouse embryo fibroblast cells. 550834). 1994; 54(22):5816-5820. Frescas D,Hall BM,Strom E,Virtuoso LP,Gupta M,Gleiberman AS,Rydkina E,Balan V,Vujcic S,Chernova OB,Gudkov AV. The cells are then passed in front of a laser beam. Science. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. No. Science. All other trademarks are the property of their respective owners. Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. XP is a registered trademark of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners. Novel role of PKR in palmitate-induced Sirt1 inactivation and endothelial cell senescence. ARF; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16, If an Invitrogen antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*. BD Pharmingen Purified Mouse Anti-Human p16. The biochemical properties of p16 suggest that it may be a tumor suppressor gene product. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. Neither the content nor the BenchSci technology and processes for 2020 Nov 25;55(11):784-790. doi: 10.3760/cma.j.cn112141-20200520-00428. will work as expected, to help you achieve results you can trust. Protect from light. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc. Members of the INK4 family of cyclin dependent kinase inhibitors include p16 INK4A, p15 INK4B, p18 INK4C, and p19 INK4D. Analyte Specific Reagent. will work as expected, to help you achieve results you can trust. T cells with aging. Gynecol Oncol. Solutions and Reagents. For flow cytometry protocols, please refer to "Intracellular Flow" at our website: http://www.bdbiosciences.com/us/s/resources. P42771. Products sold or licensed by CST Bader S,Zajac M,Friess T,Ruge E,Rieder N,Gierke B,Heubach Y,Thomas M,Pawlak M. Nrf2 status affects tumor growth, HDAC3 gene promoter associations, and the response to sulforaphane in the colon. Identification of High-Risk Human Papillomavirus DNA, p16, and E6/E7 Oncoproteins in Laryngeal and Hypopharyngeal Squamous Cell Carcinomas. For reconstitution, we recommend adding 100 L distilled water to a final antibody concentration of about 1 mg/mL. The biochemical properties of p16 suggest that it may be a tumor suppressor gene product. Results: and 1:500 Anti-CDKN2A/p16 INK4a (EPR1473, Abcam, ab209579) antibody was added and incubated at room temperature for 30 min in the dark. . 551011) offers all the necessary reagents to stain for this antibody. For analysis by flow cytometry, peritoneal cells were washed with HBSS plus 2% heat-inactivated FBS and blocked with anti-CD16/CD32 (clone 93) for 10 min on ice . Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed Staining is nuclear and/or cytoplasmic. -, Dickson MA. Cyclins and cyclin-dependent kinases (cdks) form active complexes that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. Chen YA,Tzeng DTW,Huang YP,Lin CJ,Lo UG,Wu CL,Lin H,Hsieh JT,Tang CH,Lai CH. The INK4A-ARF-INK4B locus on chromosome 9p21, frequently lost in human cancer, encodes the INK4 family members p16 INK5A and p15 INK4B, as well as the unrelated protein, ARF (1).p16 INK4A expression, typically repressed in the absence of stress, is thought to drive cells into senescence, and p16 INK4A expression is a commonly used marker of senescent cells (2,3). No. Antibodies for Flow Cytometry. 1998-2023 Abcam plc. Looks like you're visiting us from {{countryName}}. Any Customer's terms and conditions that are in Not for use in diagnostic procedures. Properties biological source mouse Quality Level 200 recombinant expressed in HEK 293 cells conjugate unconjugated antibody form purified antibody antibody product type primary antibodies clone For paraffin sections, microwave oven pretreatment with BD Retrievagen A (pH 6.0) (Cat. The p16 protein has been identified as a specific inhibitor of cdk4 because it blocks cdk4 substrate phosphorylation. A comparative analysis using abnormal cervical smears with follow-up biopsies. Ming J,Wu S,You T,Wang X,Yu C,Luo P,Zhang A,Pan X. Overexpression of ERBB4 rejuvenates aged mesenchymal stem cells and enhances angiogenesis via PI3K/AKT and MAPK/ERK pathways. lines of bacterial, yeast, or insect origin are also suitable. documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or cervical cancer; flow cytometry; human papillomavirus; immunostaining; p16INK4A; papanicolaou smear; pathology; quantification; squamous intraepithelial lesion. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala143 of human p16 INK4A protein. BMC Womens Health. miR-124 downregulation leads to breast cancer progression via LncRNA-MALAT1 regulation and CDK4/E2F1 signal activation. Carcinogenesis associated with human papillomavirus infection. 2016;11:59. Jger W,Xue H,Hayashi T,Janssen C,Awrey S,Wyatt AW,Anderson S,Moskalev I,Haegert A,Alshalalfa M,Erho N,Davicioni E,Fazli L,Li E,Collins C,Wang Y,Black PC. Unlike traditional methods for antibody production, recombinant approaches avoid the need to use animals. Nevertheless, cancer treatment may induce cellular senescence and accelerate molecular aging. (Generally, 5x10. A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. STRING - Known and Predicted Protein-Protein Interactions. NOTE: Optimal centrifugation conditions will vary depending upon cell type and reagent volume. Flow cytometric analysis of p16 expression on WI-26 VA4 human lung cells. Sponsored Products Bioss Inc. CDKN2A/p16-INK4a Polyclonal Antibody Applications: WB, ELISA, FCM, ICC, IF, IHC-fr, IHC-p Reactivity: Hu, Dg Conjugate/Tag: Cancer Res. Do not freeze. By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies (IgGs)conjugated and unconjugatedare available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western . -, Baak JPA, Kruse AJ, Robboy SJ, Janssen EAM, van Diermen B, Skaland I. For the best web browsing experience, please use Chrome, Safari or Firefox, minimum versions 77.0.3865, 12.1.2 and 68, respectively. addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized Using this assay, we were able to quantify the activity level of SA -galactosidase, the expression level of p16 INK4a and H2AX in these cell populations. These include superior lot-to-lot consistency, continuous supply, and animal-free manufacturing. The aging process implies an increase of cells expressing p16INK4a and changes in lymphocyte subpopulations. 2022 Apr 27;22(1):138. doi: 10.1186/s12905-022-01714-0. found p16 to be both nuclear and cytoplasmic in melanoma. Resuspend cells in 200-500 l of 1X PBS and analyze on flow cytometer. BMC complementary and alternative medicine, Deng S,Hu B,An HM,Du Q,Xu L,Shen KP,Shi XF,Wei MM,Wu Y. Notch1 signaling contributes to the oncogenic effect of HBx on human hepatic cells. STRING - Known and Predicted Protein-Protein Interactions. Purified Mouse IgG1 Isotype Control RUO, Biotin Goat Anti-Mouse Ig (Multiple Adsorption) RUO. The p16 protein has been identified as a specific inhibitor of cdk4 because it blocks cdk4 substrate phosphorylation. Dey A,Mustafi SB,Saha S,Kumar Dhar Dwivedi S,Mukherjee P,Bhattacharya R. Chemoproteomic Evaluation of Target Engagement by the Cyclin-Dependent Kinase 4 and 6 Inhibitor Palbociclib Correlates with Cancer Cell Response. Not for use in diagnostic or therapeutic procedures. Proceed to Permeabilization step. UniProt ID: Al-Wahab Z,Mert I,Tebbe C,Chhina J,Hijaz M,Morris RT,Ali-Fehmi R,Giri S,Munkarah AR,Rattan R. Potent effects of dioscin against liver fibrosis. -. 2005 Apr;97(1):35-40. doi: 10.1016/j.ygyno.2004.11.034. Immunohistochemistry:The p16 antibody is recommended to test for immunohistochemical staining on formalin-fixed paraffin-embedded sections. are provided for Customer as the end-user and solely for research and development uses. While polyclonal antibodies are In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. The p16 protein has been identified as a specific inhibitor of cdk4 because it blocks cdk4 substrate phosphorylation. Liu A,Guo E,Yang J,Yang Y,Liu S,Jiang X,Hu Q,Dirsch O,Dahmen U,Zhang C,Gewirtz DA,Fang H. Atorvastatin exerts inhibitory effect on endothelial senescence in hyperlipidemic rats through a mechanism involving down-regulation of miR-21-5p/203a-3p. and reliable process. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, Liang PI,Li CF,Chen LT,Sun DP,Chen TJ,Hsing CH,Hsu HP,Lin CY. Staining is nuclear and/or cytoplasmic. Moreover, since the recombinant antibody sequence is known, continuity of supply The clinical impact of using p16(INK4a) immunochemistry in cervical histopathology and cytology: an update of recent developments. Vermeer DW,Coppock JD,Zeng E,Lee KM,Spanos WC,Onken MD,Uppaluri R,Lee JH,Vermeer PD. No. Wu S,Yan W,Qiu B,Liao Y,Gu J,Wei S,Zhang A,Pan X. Antrocin Sensitizes Prostate Cancer Cells to Radiotherapy through Inhibiting PI3K/AKT and MAPK Signaling Pathways. A cell cycle regulator potentially involved in genesis of many tumor types. and reliable process. Cell . p16 INK4A protein expression is often altered in human cancer, and high expression is currently used as a predictive biomarker in cervical cancer (3-5). For the best web browsing experience, please use Chrome, Safari or Firefox, minimum versions 77.0.3865, 12.1.2 and 68, respectively. Alternatively, cells may be stored overnight at 4C in 1X PBS. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. P16/Ki-67 Dual Staining in Positive Human Papillomavirus DNA Testing for Predictive Diagnosis of Abnormal Cervical Lesions in Northeastern Thai Women. American journal of physiology. Since 2018, 24 100-women (HPV-positive/-negative, Pap-normal/-abnormal) have been enrolled nationwide for two-tier validation work. When the p16 antibodies were no longer available from Santa Cruz, we used a different antibody (NA29, 1:100) from EMD Millipore (Billerica, MA). Is p16(INK4A) expression more useful than human papillomavirus test to determine the outcome of atypical squamous cells of undetermined significance-categorized Pap smear? Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated Mammalian cell lines are most commonly used for recombinant antibody production, although cell Song FB, Du H, Xiao AM, Wang C, Huang X, Yan PS, Liu ZH, Qu XF, Belinson JEROMEL, Wu RF. For research use only Product format Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide. The authors declare no conflicts of interest. Alternative reading frame; ARF; CDK4 inhibitor p16 INK4; CDK4 inhibitor p16-INK4; CDK4I; cell cycle negative regulator beta; Cyclin dependent kinase 4 inhibitor A; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); HGNC:1787; inhibits CDK4; isoforms 1/2/3; Melanoma p16 inhibits CDK4; MTS-1; Multiple tumor suppressor 1; P14 P16; p14ARF; p16-INK4; p16-INK4a; P19 TP16; Tumor suppressor ARF, Gene Aliases: 1994; 54(23):6078-6082. FCM-based p16INK4A quantification offers a better choice for conveniently and precisely monitoring the occurrence of HSIL+ and directing risk-stratification-based interventions. Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use. Alternatively, the Anti-Mouse Ig HRP Detection Kit (Cat. Overexpression of PP1-NIPP1 limits the capacity of cells to repair DNA double-strand breaks. In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic Alternatively, cells may be stored overnight at 4C in 1X PBS. Background: The fluorescent probe Syto16 has been used successfully to measure P-glycoprotein (Pgp) function and, separately, early apoptosis and cell death.